Photoactive probes and reactivity for highly selective analyses and imaging

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Photoactive probes and reactivity for highly selective analyses and imaging

When practiced at its interface with biology and physics, chemistry offers more than a toolkit for labeling biomolecules or analyzing cellular composition. It provides a distinct molecular perspective that enables quantitative interrogation of interactions and kinetic analysis of exquisitely complex reaction networks.

This perspective underlies our research activity: we introduce and implement chemical concepts and tools to interrogate and manipulate biological systems. More specifically, our recent work focuses on the development of photoactive organic probes and reactivity-based protocols for highly selective analyses and imaging in live cells as well as in photosynthetic organisms (microalgae and plants).

Recent References

  • R. Chouket, A. Pellissier-Tanon, A. Lahlou, R. Zhang, D. Kim, M.-A. Plamont, M. Zhang, X. Zhang, P. Xu, N. Desprat, D. Bourgeois, A. Espagne, A. Lemarchand, T. Le Saux, L. Jullien,
    Extra kinetic dimensions for label discrimination, Nature Communications, 2022, 13, 1482.

  • A. Lahlou, H. Sepasi Tehrani, I. Coghill, Y. Shpinov, M. Mandal, M.-A. Plamont, I. Aujard, Y. Niu, L. Nedbal, D. Lazár, P. Mahou, W. Supatto, E. Beaurepaire, I. Eisenmann, N. Desprat, V. Croquette, R. Jeanneret, T. Le Saux, L. Jullien,
    Nature Methods, 2023, 20(12), 1930–1938.

  • H. Merceron, I. Coghill, A. Lahlou, M.-A. Plamont, L. Jullien, T. Le Saux,
    Periodic Light Modulations for Low-Cost Wide-Field Imaging of Luminescence Kinetics Under Ambient Light, Advanced Science, 2025, 12(10), 2413291.

  • H. Merceron, E. Israelievitch, V. Rollot, T. Villarubias, X. Xie, T. Le Saux, K. Benzerara, F. Guyot, A. Boulouis, E. Marie-Bègue, L. Thouin, L. Jullien,
    A Reaction–Diffusion Frame for Accessing Metabolic O₂ Fluxes in Single Microalgal Cells with Low-Cost Wide-Field Imaging of Nanosensor Luminescence Lifetime, Advanced Science, 2025, 12(42), e10903.

  • Y. Shpinov, M. Mandal, V. van Deuren, A. Lahlou, M. Le Bec, R. Chouket, C. Hadj Moussa, C. Bonin, H. Sepasi Tehrani, I. Coghill, L. El Hajji, K. Ounoughi, J. Franco Pinto, M.-A. Plamont, P. Pelupessy, I. Ayala, F. Perez, I. Aujard, T. Le Saux, A. Gautier, P. Dedecker, B. Brutscher, L. Jullien,
    Photoejection turns non-covalent fluorescent tags into negative reversible photoswitchers, bioRxiv, 2025, doi: 10.1101/2025.12.05.692574.